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. 2014 Sep 22;9(9):e107164. doi: 10.1371/journal.pone.0107164

Figure 10. Apoptotic changes in mitochondria.

Figure 10

Representative illustration of the depolarization of mitochondrial transmembrane potential of sperm cells by DGT, measured by lipophilic cationic dye JC-1 staining. Concentrations used were 29.01 µM, 58.03 µM, and 106.38 µM against sperm for 3 h. [A. control, medium only, B. 29.01 µM DGT, C. 58.03 µM DGT, D. 106.38 µM DGT, E. CCCP]. Cell population in quadrants identified as: [upper right, polarized; lower right, depolarized].