Figure 7. BPAG1 knockdown leads to a reduction in dextran uptake and/or retention within the C2.7 myoblasts.

A) Sparsely seeded cells were incubated with FITC-dextran (green) for 10 min and then chased for 1 h. Afterward cells were fixed in PFA, stained with DAPI (blue), and mounted. Scale bar: 20 µm. B) Graph represents quantification of FITC signal that was measured using ImageJ for each individual cell (84–104 cells/condition). CTCF (corrected total cell fluorescence)  =  Integrated Density – (Area of selected cell × Mean fluorescence of background readings). Data are mean ± SEM, *p = 0.03 (n = 3 independent experiments).