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. 2014 Sep 22;9(9):e107774. doi: 10.1371/journal.pone.0107774

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© 2014 Fan et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Caspase-3 and Bcl-2 immunoreactivity were observed in the cell cytoplasm of cartilage, trabecular bone and bone marrow. (A). In the model group, relatively high immunoreactivity of caspase-3 was observed. (B). In the prevention group, weak immunoreactivity of caspase-3 was found. (C) and (F). Bar graphs represent the mean optical density of caspase-3 and Bcl-2 in bone marrow. Semi-quantitative analysis was based on at least 10 fields per section. The asterisk (*) shows statistically significant differences between the two groups. (D). In the model group, low immunoreactivity of Bcl-2 was observed. (E). In the prevention group, higher immunoreactivity of Bcl-2 was found.