. 2014 Sep 22;9(9):e107494. doi: 10.1371/journal.pone.0107494

Table 2. Antibody reagents for immunofluorescence detection of dystrophin and spectrin.

Double-staining combination	Dystrophin primary antibody	Spectrin primary antibody	Secondary antibody antimouse	Secondary antibody antirabbit
MANDYS106 and spectrin	Mouse MANDYS106 [9] ^a	Rabbit α-spectrin (Thermo Scientific PA1-46007)^c	α-mouse AlexaFluor488 (Invitrogen A11029)^d	α-rabbit AlexaFluor594 (Invitrogen A11037)^e
Mouse isotype and spectrin	Mouse IgG2A (Sigma M5409)^b	Rabbit α-spectrin (Thermo Scientific PA1-46007)^c	α-mouse AlexaFluor488 (Invitrogen A11029)^d	α-rabbit AlexaFluor594 (Invitrogen A11037)^e
ab15277 and spectrin	Rabbit polyclonal (Abcam ab15277)^c	Mouse α-spectrin (Novocastra NCL-SPEC1)^c	α-mouse AlexaFluor594 (Invitrogen A11032)^e	α-rabbit AlexaFluor488 (Invitrogen A11034)^d
Rabbit isotype and spectrin	Rabbit polyclonal IgG (Abcam ab27478)^c	Mouse α-spectrin (Novocastra NCL-SPEC1)^c	α-mouse AlexaFluor594 (Invitrogen A11032)^e	α-rabbit AlexaFluor488 (Invitrogen A11034)^d

Dilution of primary antibodies in PBS containing 0.05% Tween, 5% FBS: ^a1∶60,^b1∶300, ^c1∶200.

Dilution of secondary antibodies in PBS containing 0.05% Tween: ^d1∶250,^e1∶1000.

FBS, fetal bovine serum; Ig, immunoglobulin; PBS, phosphate-buffered saline.