Table 5. Summary of experiments: different operators performing the staining, imaging, and Definiens image processing.
Experiment | Intensity of control beads (% laser intensity) | Operators | ||
Staining | Image acquisition | Definiens processing | ||
7 | 2302 (7%) | 1, 9 | 4, 8 | 8, 9, 4, 10 |
6 | 2137 (7%) | 1, 3 | 2, 4 | 3, 6, 7 |
5 | 2076 (7%) | 1, 2 | 3, 4 | 3, 4 |
4 | 1887 (7%) | 2, 6 | 5, 7 | 1 |
3 | 2295 (7%) | 5, 6 | 4, 3 | 3, 6, 1 |
2 | 340 (1%) | 3, 5 | 1, 4 | 1, 3 |
1 | N/D | 3 | 3, 4 | 3, 4, 5 |
N/D, not determined.