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. 2014 Sep 22;9(9):e108182. doi: 10.1371/journal.pone.0108182

Figure 3. Effects of high glucose, bFGF and FGFR1 inhibitor on fibroblast cell migration and JNK phosphorylation.

Figure 3

(A) Wound healing assay was performed to analyze effects of HG, 100 ng/mL of bFGF (b) and 50 nM of FGFR1 inhibitor PD173074 (PD) in human fibroblasts and mannitol was used as an osmotic control (5.5 mM glucose plus 24.5 mM mannitol) and LG means 5.5 mM glucose in the culture medium. (B) Cell migration distance was measured according to the data shown in (A). Data represent mean values ±SE of 10 replicates, as compared to the HG group (*P<0.001, t test). (C) Phosphorylation levels of AKT and JNK were analyzed after 30 min of HG, HG+bFGF (b) and HG+bFGF+PD173074 (PD) stimulation. All experiments were performed after 5 µg/mL mitomycin-C (cell proliferation inhibitor) application for one day. Densitometry for p-AKT (D) and p-JNK (E) was normalized to the amount of total AKT and JNK. The results are presented as fold change as compared to fibroblasts grown in the 5.5 mM glucose (LG) containing medium. Data represent mean values ±SE of three independent experiments (*P<0.05, t test).