Skip to main content
. 2014 Apr 17;5(15):5934–5949. doi: 10.18632/oncotarget.1904

Figure 5. Gamma-secretase inhibitor prevents nuclear translocation and reverses resistance in trastuzumab-resistant cells.

Figure 5

(A & B) SKBR3 cells were treated with 10μM γ-secretase inhibitor (GSi) with or without 40μg/ml trastuzumab before performing western blot analysis and confocal microscopy to investigate HER4 expression by IHC and localization by confocal microscopy. (C) Cell count and apoptosis assays were performed after treatments as in (A) and (B). Means ± SD from three independent experiments are shown (***p<0.001). (D) Figures show the representative HER4 immunoblots on cytoplasmic and nuclear fractions from trastuzumab-resistant SKBR3 cells treated with GSi. (E) The effect of GSi on trastuzumab-resistant SKBR3 was studied using colony formation assay. Representative colony pictures are shown on the upper panel and the relative ratio of colony counts are shown on the bottom panel.