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. 2014 Apr 17;5(15):5950–5964. doi: 10.18632/oncotarget.1909

Figure 4. Impact of hydralazine on specific gene methylation of PCa cells.

Figure 4

(A) Real-time RT-PCR of mRNA levels of DNMT1, DNMT3a and DNMT3b normalized to GUSB in vehicle and drug exposed cells. (B) Western blot analysis of DNMT1 protein expression. β-Actin was used as a loading control. (C) Real-time methylation-specific PCR (qMSP) for assessment of GSTP1, APC, RARβ2, CCND2 and BCL2 methylation levels, normalized to β-Actin. (D) mRNA expression of GSTP1, APC, RARβ2, CCND2 and BCL2, by real-time RT-PCR, in both hydralazine and vehicle exposed cells. All data are presented as mean of three independent experiments ± s.d.