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. 2014 Jul 7;5(15):6113–6129. doi: 10.18632/oncotarget.2176

Figure 1. Effect of miR-451 expression on in vitro radiosensitivity of docetaxel-resistant LAD cells.

Figure 1

(A) qRT-PCR detection of relative miR-451 expression in docetaxel-resistant LAD cells (SPC-A1/DTX and H1299/DTX) and their parental LAD cells (SPC-A1 and H1299). U6 was used as an internal control. (B) qRT-PCR detection of relative miR-451 expression in pcDNA/miR-451(or pcDNA/miR-NC)-transfected SPC-A1/DTX or H1299/DTX cells, and Anti-miR-451 (or Anti-miR-NC)-transfected SPC-A1 or H1299 cells, respectively. U6 was used as an internal control. (C) CCK-8 assay was conducted to detect the ED50 values of irradiation to pcDNA/miR-451(or pcDNA/miR-NC)-transfected SPC-A1/DTX or H1299/DTX cells. (D) CCK-8 assay was conducted to detect the ED50 values of irradiation to Anti-miR-451 (or Anti-miR-NC)-transfected SPC-A1 or H1299 cells. (E) The colony formation of pcDNA/miR-451(or pcDNA/miR-NC)-transfected SPC-A1/DTX or H1299/DTX cells treated without irradiation or with irradiation (4.0 Gy). (F) The colony formation of Anti-miR-451 (or Anti-miR-NC)-transfected SPC-A1 or H1299 cells treated without irradiation or with irradiation (2.0 Gy). Results represent the average of three independent experiments (mean±SD). *P<0.05 and **P< 0.01.