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. 2014 Jul 7;5(15):6113–6129. doi: 10.18632/oncotarget.2176

Figure 3. Effect of miR-451 expression on irradiation-mediated DNA DSBs of docetaxel-resistant LAD cells.

Figure 3

(A) Immunofluorescence detection of phosphorylation of H2A.X (γ-H2A.X) foci formation (a marker of DSB) in pcDNA/miR-451(or pcDNA/miR-NC)-transfected SPC-A1/DTX or H1299/DTX treated without irradiation or with irradiation (4.0 Gy). (B) Western blotting detection of γ-H2A.X protein expression in pcDNA/miR-451(or pcDNA/miR-NC)-transfected SPC-A1/DTX or H1299/DTX treated without irradiation or with irradiation (4.0 Gy). GAPDH was used as an internal control. Results represent the average of three independent experiments (mean±SD). **P< 0.01.