Figure 6. Aurora-A inhibition induces mis-oriented cell division.

A. An example of normally dividing cell is shown on top. Mis-oriented division in the presence of MLN8237 in U2OS cells with fluorescently labeled H2B (green) and alpha-tubulin (red) is shown below. Minutes from round-up are indicated. Scale bars: 10 μm. B. Quantification (%) of mis-oriented cell division recorded from MLN8237 treatment start for the following 20 hours (80-200 mitoses per condition from 3-4 independent experiments). *: 0.01<p<0.02; **: p< 0.01, χ² test. C. The angle between the growth surface and the centrosome-centrosome axis in MLN8237-treated mitoses is calculated as schematized on top (centrosomes are in red; see Methods for details); histograms represent the distribution of prometaphases in 3 classes (50-60 cells from 3 independent experiments).