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. 2014 Jul 9;5(15):6267–6279. doi: 10.18632/oncotarget.2195

Figure 2. STAT3 is strongly activated in breast cancer cells, following stimulation with Wound Fluids.

Figure 2

(A) Table reports the activation of five different members of STAT family in MDA-MB-231 cells, following stimulation with 5% wound fluids for 20 minutes (stimulated) or not (unstimulated). Activation was detected using a commercial immunoassay. The value of “fold induction” represents the ratio between the unstimulated and stimulated values. (B) Western blot analysis of MDA-MB-468 and MDA-MB-231 cell lines serum starved and then stimulated for the indicated times with 10% serum (FBS) or 5% wound fluids (WF). (C) Same as in (A), but using MDA-MB-453 and SK-BR-3 cell lines, as indicates. (D) Same as in (A), but using MCF-7 and BT-474 cell lines, as indicated. Vinculin expression was used as loading control.