Figure 2. Effect of PTEN expression or PI3K inhibition on cPLA₂α protein levels.

(a) LNCaP cells carrying tet repressor were stably transfected with either Dox-inducible PTEN (LNCaP-i-PTEN) or the empty vector (LNCaP-i-Empty), and treated with or without Dox (100 ng/mL) for 24 h. The harvested cells were lysed and supernatants were used for immunoblot. (b) Quantified band intensity of 3 independent experiments is represented as the mean ± SD. * P<0.05 versus no Dox treatment. (c) PC-3 cells stably transfected with PTEN or empty vector were lysed for immunoblot. (d) Quantified band intensity of 3 independent experiments is represented as the mean ± SD. * P<0.05 versus empty vector-transfected cells. (e) PC-3 cells treated with PI3K inhibitor (LY294002) for 1 h at indicated doses were harvested at 24 h for immunoblot. (f) Quantified band intensity of 3 independent experiments is represented as the mean ± SD. * P<0.05 versus vehicle-treated control.