Skip to main content
. 2014 Jul 23;5(15):6484–6496. doi: 10.18632/oncotarget.2248

Figure 5. CK2 Inhibition Affects the Phosphorylation State and Transcriptional Capacity of TNF-α-induced NF-κB.

Figure 5

A, MDA-MB-231 cells were treated for 4 h in serum-free medium with CX-4945 at 2, 5 and 10 μM and then treated for 15 minutes with 1 ng/mL of TNF-α. Lysates were obtained, and immunoblots performed using the indicated antibodies. Densitometric quantifications for each phosphoprotein are shown, with cells treated with cytokine alone serving as the reference point (100%); percent inhibition is shown. B, MDA-MB-231 cells were treated for 4 h in serum-free medium with CX-4945 at 2, 5 and 10 μM and then treated for 1 h with 1 ng/mL of TNF-α. RNA was isolated, cDNA was generated, and q-RTPCR was performed for the indicated genes. PCR was performed in triplicate: **, p<0.001. C, MDA-MB-231 cells were treated for 48 h with 1 ng/mL of TNF-α and/or 10 μM of CX-4945. Supernatants were collected, and ELISA was performed for the indicated cytokines. ELISA performed in triplicate: *, p≤0.01; **, p<0.001; ***, p<0.0001. All experiments were repeated, and representative data are shown.