Table 1.
Flow cytometry staining panels used to monitor abatacept study
| ITN panel no. | Fluorochrome and cell markers |
Main subpopulations identifiable | ||||
|---|---|---|---|---|---|---|
| FITC | PE | APC | PE-Cy5.5 | PE-Cy7 | ||
| 7 | CD45RA | CD45R0 | CD62L | CD8 | CD4 | CD4, CD8-naive, and memory subsets |
| 54 | CD11c | CD80-BMS | DUMP | HLA-DR | CD123 | Myeloid and plasmacytoid dendritic cell subsets |
| 55 | CD11c | CD86-BMS | DUMP | HLA-DR | CD123 | Myeloid and plasmacytoid dendritic cell subsets |
| 57 | CD14 | CD80-BMS | CD19 | CD3 | CD86-BMS | Monocytes, B cells, T cells |
| 122 | CD62L | CD25 | CD8 | CD4 | CD45R0 | Tregs (CD62L+CD25highCD8−CD4+CD45R0+) |
| 123 | 6B11* | Vα24 | CD8 | CD4 | CD69 | Invariant NK T cells |
| 124 | CD62L | CD25 | CD8 | CD4 | CTLA-4/CD152 | Tregs (CD62L+CD25highCD8−CD4+CD152+) |
| 131 | CD2 | CD69 | CD8 | CD4 | CD45R0 | Activated (CD69+) CD4 and CD8 T cells |
APC, antigen-presenting cell; FITC, fluorescein isothiocyanate.
*Clone recognizes invariant CDR3 region of TCR Vα24-JαQ.