Figure 2.

Influence of CuE on cell cycle progression/distribution in Detroit 562 and Hone-1 cells: (A) Cell cycle analysis of Detroit 562 and Hone-1 cells after being cultured with CuE for 24 h. (B) CuE induced an increase in G₂/M phase cells (%). (C) MPM-2 (anti-phospho-Ser/Thr-Pro) expression in untreated and treated cancer cells. MPM-2 is an antibody that recognizes proteins which are only phosphorylated in mitosis. Cells were dually stained using propidium iodide to analyze DNA content and protein expression was quantified by flow cytometry. As a positive control, separate groups of cells were treated for 24 h with nocodazole (15 μg/mL), an anti-fungal agent known to induce metaphase arrest. Cell-cycle analysis and quantification of MPM-2 expression (gated cells) were performed by flow cytometry following treatment with CuE for 24 h. (D) CuE enhanced the level of MPM-2 in Detroit 562 and Hone-1 cells. Symbol (*) in each group of bars indicates that the difference resulting from treatment with CuE 0 μM is statistically significant at P < 0.05.