Table 2.
Various alkaline phosphatase (AP) substrates used with this in situ PCR protocol on different plant species and tissues
| Plant species | Tissue | AP substrate |
|---|---|---|
|
Arabidopsis thaliana |
Leaf |
BM Purple |
| Elf97 | ||
| Vector Blue | ||
|
Arabidopsis thaliana |
Flower |
BM Purple |
|
Cleome gynandra |
Leaf |
BM purple |
|
Cleome hassleriana |
Leaf |
BM purple |
|
Glycine max |
Root |
BM purple |
|
Hordeum vulgare |
Leaf |
BM purple |
| Elf97 | ||
|
Hordeum vulgare |
Epidermal peel |
BM Purple |
| Elf97++ | ||
|
Oryza sativa |
Leaf |
BM Purple |
| Vector Blue | ||
|
Oryza sativa |
Root |
BM Purple |
|
Solanum lycopersicum |
Leaf |
Vector Blue |
|
Triticum aestivum |
Root |
BM Purple |
|
Triticum durum |
Leaf |
Elf97 |
|
Triticum durum |
Root |
BM Purple |
| NBT/BCIPX | ||
| Vector Blue++ | ||
|
Vitis vinifera |
Root |
Vector Blue |
|
Vitis vinifera |
Leaf |
BM Purple |
| Zea mays | Leaf | BM Purple |
Most AP substrates were used successfully for specific detection of the transcript in question, with some exceptions as indicated. ++indicates detection with significant background staining. Xindicates no detectable signal.