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RT reaction solution
| Component | Stock conc. | Final conc./amount | 1× (μL, in-tube) | 1× (μL, on-slide) |
|---|---|---|---|---|
| cDNA synthesis buffer (kit) |
5× |
1× |
4 |
20 |
| DTT (kit) |
0.1 M |
5 mM |
1 |
5 |
| RNaseOUT (kit) |
40 U μL−1 |
40 U |
1 |
1 |
| Thermoscript RT (kit) | 15 U μL−1 | 15 U | 1 | 1 |
