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Rhamnogalacturonan-II (RG-II) largely fails to dimerise in Rosa culture apoplast. Purified monomeric RG-II (about 60 μm) was incubated in cell-free spent medium harvested from a zero-B Rosa culture 4 days after subculture. (a) No H3BO3, (b) H3BO3 added to 1.2 mm concurrently with the RG-II, (c) cells and 1.2 mm H3BO3 added. At intervals (0–24 h), samples of the medium were frozen and later electrophoresed.
