Table 1.
β-catenin heterozygous knockout mice do not respond to loading. Data represent mean ± SD for bone formation rate/bone surface (BFR/BS), bone formation rate/bone volume (BFR/BV), bone formation rate/total volume (BRF/TV), single labeled surface per bone perimeter (sL.S/B.Pm), and double labeled surface per bone perimeter (dL.S/B.Pm) in the endocortical and periosteal region. Group size n=5 for male controls and HET cKO, n=4 for female controls and HET cKO. a = statistically significant different comparing male to female; b = statistically significantly different comparing control versus HET cKO; and c = statistically significant different between non loaded and loaded ulna. Statistical significance was determined using a two independent sample t-test (control and HET cKO) and matched paired t-test for left and right (load, non-loaded).
| Male |
Female |
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|---|---|---|---|---|---|---|---|---|---|
| Control | HET cKO | Control | HET cKO | ||||||
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| Non-Loaded | Loaded | Non-Loaded | Loaded | Non-Loaded | Loaded | Non-Loaded | Loaded | ||
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| Endocortical | BFR/BS (mcm3/mcm2/d) | 0.01 ± 0.01 | 1.43 ± 0.19 b,c | ND | 0.03 ± 0.02 | 0.01 ± 0.01 | 1.71 ± 0.36b,c | 0.03 ± 0.02 | 0.05 ± 0.05 |
| BFR/BV (%/y) | 0.01 ± 0.01 | 0.35 ± 0.06b,c | ND | 0.01 ± 0.01 | ND | 0.63 ± 0.14b,c | 0.01 ± 0.01 | 0.01 ± 0.01 | |
| BFR/TV (%/y) | ND | 0.32 ± 0.05 b,c | ND | 0.01 ± 0.1 | ND | 0.54 ± 0.11b,c | 0.01 ± 0.01 | 0.01 ± 0.01 | |
| Ec.sL.S/B.Pm (%) | 14.0 ± 10.3 | 51.40 ± 13.50 | 7.48 ± 6.83 | 8.80 ± 8.30 | 12.09 ± 4.29 | 47.01 ± 4.96 | 7.60 ± 1.30 | 5.97 ± 0.54 | |
| Ec.dL.S/B.Pm (%) | 1.80 ± 4.10 | 75.50 ± 35.70 | ND | 4.30 ± 4.90 | 1.12 ± 1.94 | 70.31 ± 26.45 | 3.72 ± 5.24 | 6.46 ± 10.19 | |
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| Periosteal | BFR/BS (mcm3/mcm2/d) | 0.01 ± 0.01 | 0.49 ± 0.13a,b,c | ND | 0.07 ± 0.06 | ND | 1.57 ± 0.16b,c | 0.02 ± 0.02 | 0.08 ± 0.05 |
| BFR/BV (%/y) | 0.01 ± 0.01 | 0.44 ± 0.13a,b,c | ND | 0.07 ± 0.05 | ND | 1.55 ± 0.15b,c | 0.02 ± 0.02 | 0.07 ± 0.04 | |
| BFR/TV (%/y) | 0.01 ± 0.01 | 0.39 ± 0.11a,b,c | ND | 0.06 ± 0.05 | ND | 1.33 ± 0.14b,c | 0.01 ± 0.02 | 0.06 ± 0.04 | |
| Ps.sL.S/B.Pm | 5.44 ± 4.44 | 43.36 ± 9.75 b,c | 1.78 ± 2.05 | 8.58 ± 3.19 | 4.46 ± 3.51 | 35.83 ± 5.15b,c | 0.70 ± 0.72 | 5.71 ± 5.14 | |
| Ps.dL.S/B.Pm (%) | 1.52 ± 3.41 | 25.09 ± 7.94b,c | ND | 3.35 ± 4.07 | ND | 33.33 ± 7.67b,c | 1.64 ± 3.27 | 5.21 ± 6.19 | |