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a) Paramagnetic relaxation as a function of Gd(DTPA-BMA) concentration. The increase of R1 is shown for selected protons of the 42 kDa maltose-binding protein (MBP). Relaxation rates were measured by using a saturation-recovery scheme, preceding an HSQC-based detection block. b) The residues for which R1 enhancements are shown in a) are labelled. All available experimental PREs are mapped onto the structure of MBP (red=low PRE, blue=high PRE).[4b]
