Table 1.
Fragment amplified region | Amplicon length (bp) | Sequence (5′→3′) | Primer length (bp) | Annealing temperature (°C) |
---|---|---|---|---|
Promoter-Exon 1 | 743 | CCAACGCCTATAACAACTCATCC | 23 | 58 |
TCAGTCAAACGTGCACTCACAG | 22 | |||
Exon 2 | 835 | GCCATAGGTGCGTAAATCGTGCGT | 24 | 64 |
CCTCTCAGCAACCCCCAGAACCTC | 24 | |||
Exon 3 | 671 | CCTTGCTCTTTACATGCTCGCTCAT | 25 | 64 |
GCCGCCAAAGGTGCTTCAACATAG | 24 |