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. 2014 Aug 5;45(5):709–715. doi: 10.1111/age.12202

Table 1.

PCR primer sets used for amplification of the promoter to exon 3 of the IGF1 gene in Atlantic salmon.

Fragment amplified region Amplicon length (bp) Sequence (5′→3′) Primer length (bp) Annealing temperature (°C)
Promoter-Exon 1 743 CCAACGCCTATAACAACTCATCC 23 58
TCAGTCAAACGTGCACTCACAG 22
Exon 2 835 GCCATAGGTGCGTAAATCGTGCGT 24 64
CCTCTCAGCAACCCCCAGAACCTC 24
Exon 3 671 CCTTGCTCTTTACATGCTCGCTCAT 25 64
GCCGCCAAAGGTGCTTCAACATAG 24