Table 3.
Average Ct values ± SD of the seven reference genes in the different experimental setups.
| Treatment | EEF1A | RPL8 | TUBA | B2MG | ACTB | G6PDH | GAPDH | |
|---|---|---|---|---|---|---|---|---|
| Drug | Azithromycin | 20.49 ± 0.78 | 20.86 ± 0.59 | 23.90 ± 0.37 | 29.78 ± 1.84 | 27.22 ± 0.43 | 25.87 ± 0.32 | 27.97 ± 0.52 |
| Ivermectin | 20.88 ± 0.66 | 21.05 ± 0.93 | 24.34 ± 1.58 | 29.23 ± 0.45 | 27.53 ± 1.10 | 27.21 ± 0.12 | 29.13 ± 0.99 | |
| Florfenicol | 19.86 ± 0.19 | 20.57 ± 0.50 | 23.87 ± 0.37 | 29.19 ± 1.05 | 27.22 ± 0.15 | 26.91 ± 0.52 | 28.54 ± 0.35 | |
| Norfloxacin | 19.40 ± 0.51 | 19.28 ± 0.58 | 22.23 ± 0.46 | 26.52 ± 0.98 | 25.80 ± 0.46 | 24.91 ± 0.55 | 26.21 ± 0.69 | |
| Levamisole | 18.73 ± 0.57 | 18.63 ± 0.53 | 21.97 ± 0.24 | 25.12 ± 0.41 | 25.46 ± 0.56 | 25.52 ± 0.90 | 26.28 ± 1.02 | |
| Ceftriaxone | 18.73 ± 0.13 | 18.68 ± 0.17 | 21.38 ± 0.23 | 25.36 ± 0.92 | 24.72 ± 0.11 | 25.15 ± 0.15 | 25.53 ± 0.39 | |
| Rifampicin | 18.71 ± 0.55 | 18.65 ± 0.59 | 21.49 ± 0.48 | 26.83 ± 1.02 | 25.11 ± 0.61 | 25.41 ± 0.69 | 25.95 ± 0.75 | |
| Control | 18.59 ± 0.74 | 18.57 ± 0.62 | 22.24 ± 0.86 | 27.90 ± 1.25 | 25.79 ± 0.77 | 26.45 ± 0.48 | 27.06 ± 1.18 | |
| 16 | One-year-old male | 19.29 ± 0.39 | 19.99 ± 0.22 | 21.94 ± 0.44 | 23.61 ± 0.80 | 25.47 ± 0.43 | 25.09 ± 0.72 | 27.04 ± 1.64 |
| Two-year-old male | 20.25 ± 0.04 | 21.10 ± 0.04 | 22.71 ± 0.06 | 24.54 ± 0.91 | 26.44 ± 0.22 | 26.96 ± 0.30 | 28.09 ± 0.97 | |
| One-year-old female | 19.79 ± 0.61 | 20.14 ± 0.92 | 22.50 ± 0.59 | 23.53 ± 0.62 | 25.60 ± 0.40 | 26.21 ± 1.21 | 26.93 ± 1.37 | |
| Two-year-old female | 19.75 ± 0.69 | 20.07 ± 1.00 | 22.53 ± 0.73 | 23.57 ± 0.94 | 25.90 ± 0.65 | 26.02 ± 0.54 | 27.42 ± 0.53 | |
| 26 | One-year-old male | 18.81 ± 0.31 | 19.59 ± 0.37 | 22.34 ± 0.52 | 23.24 ± 0.59 | 25.62 ± 0.55 | 26.41 ± 1.00 | 27.32 ± 1.31 |
| Two-year-old male | 19.73 ± 0.40 | 20.26 ± 0.63 | 23.33 ± 0.83 | 23.64 ± 0.49 | 26.73 ± 1.22 | 27.03 ± 1.13 | 27.69 ± 1.10 | |
| One-year-old female | 18.87 ± 0.34 | 19.48 ± 0.49 | 22.79 ± 0.79 | 23.17 ± 0.12 | 26.03 ± 0.53 | 26.43 ± 0.95 | 27.37 ± 1.11 | |
| Two-year-old female | 19.37 ± 0.06 | 19.96 ± 0.15 | 23.36 ± 0.18 | 23.86 ± 0.88 | 26.59 ± 0.32 | 26.27 ± 0.42 | 28.15 ± 0.34 | |
| maximum | 20.88 ± 0.66 | 21.10 ± 0.04 | 24.34 ± 1.58 | 29.78 ± 1.84 | 27.53 ± 1.10 | 27.21 ± 0.12 | 29.13 ± 0.99 | |
| minimun | 18.59 ± 0.74 | 18.57 ± 0.62 | 21.38 ± 0.23 | 23.17 ± 0.12 | 24.72 ± 0.11 | 24.91 ± 0.55 | 25.53 ± 0.39 |