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. 2013 Jul 23;30(9):341–351. doi: 10.1002/yea.2967

Table 1.

Aid-tagging plasmids used in this study

Name IAA17 Position Extension Selection Parent vector
pHyg–AID1–114 1–114 C-terminal – hphNT pSM409
pHyg–AID31–114 31–114 C-terminal – hphNT pSM409
pHyg–AID* 71–114 C-terminal – hphNT pSM409
pHyg–AID1–114–8myc 1–114 C-terminal 8myc hphNT pSM409
pHyg–AID31–114–9myc 31–114 C-terminal 9myc hphNT pSM409
pHyg–AID*–9myc 71–114 C-terminal 9myc hphNT pSM409
pHyg–AID*–6FLAG 71–114 C-terminal 6FLAG hphNT pSM409
pHyg–AID*–6HA 71–114 C-terminal 6HA hphNT pSM409
pHyg–AID*–GFP 71–114 C-terminal GFP hphNT pSM409
pKan–AID*–9myc 71–114 C-terminal 9myc kanMX pSM409
pNat–AID*–9myc 71–114 C-terminal 9myc natNT pSM409
pHis–AID*–9myc 71–114 C-terminal 9myc HIS3MX pSM409
pKan–PCUP1–AID*(N) 71–114 N-terminal – kanMX pMK38
pKan–PCUP1–AID*(N)–9myc 71–114 N-terminal 9myc kanMX pMK38
pKan–PCUP1–9myc–AID*(N) 71–114 N-terminal 9myc kanMX pMK38
pKan–PRFA1–9myc–AID*(N) 71–114 N-terminal 9myc kanMX pMK38