Table 1.
Micropanning assay to determine binding of selected phage clones to SKOV-3 and HS-832 cells
| Phage No. | Peptide sequence | SKOV-3 to HS-832 ratio |
|---|---|---|
| pJ1 | RTEVPVLSFTSPLTG | 1.76 |
| pJ2 | GDVWLFKTSTSHFAR | 1.91 |
| pJ3 | AREYGTRFSLIGGYR | 0.21 |
| pJ4 | HAAFEPRGDVRHTLL | 2.00 |
| pJ5 | LGRAGQSYPSFARGL | 0.52 |
| pJ6 | PIFPVVSSSGSSSSP | 1.58 |
| pJ7 | PLSHGSVVYPRSSLG | 1.36 |
| pJ8 | RRDTVPRSLSAPLSW | 0.59 |
| pJ9 | PAVASTSSLIIDGPF | 2.29 |
| pJ10 | HPPLASVWHVSVPL | 0.83 |
| pJ11 | LHDFRSPIYASLLGF | 1.53 |
| pJ12 | AGDGGLGRVAAGARV | 1.15 |
| pJ13 | RVFHLWPHPTSTLSA | 0.25 |
| pJ14 | APLSYNFASMPFMSG | 0.73 |
| pJ15 | HPGWFDSAWFRAVSR | 1.24 |
| pJ16 | ARDSRCGGFLGCGVT | 1.36 |
| pJ17 | AMVRGFSFGMSRGSD | 1.94 |
| pJ18 | RSLWSDFYASASRGP | 6.57 |
| pJ19 | SYSVVNSPWCDGTCD | 1.25 |
| pJ20 | SRDGLHSFCYVGCPP | 0.40 |
| pJ21 | GVGDADGFIPVISAV | 0.59 |
| pJ22 | PVFFRLSPVTEGGGV | 1.40 |
| pJ23 | FPSYPFIAYSLQTPV | 1.23 |
| pJ24 | RRLPHLMPFEGSVFL | 3.55 |
| pJ25 | GPHFDYRTGLGWRFG | 1.35 |
| pJ26 | LGKGLTGSALSLSAL | 2.29 |
| pJ27 | YGVTPSPRSPWATAH | 3.43 |
| pJ28 | VFVDGARYSTASDSL | 2.83 |
| pJ29 | GAGIFGPWGVFAAVP | 1.21 |
| pJ30 | GYRSAFVPFVARGGH | 3.65 |
| pJ31 | RYRVGFTPGTIAAVL | 1.13 |
SKOV-3 or HS-832 cells were incubated with individual phage clones and eluted using 2.5% CHAPS. Phage binding was determined by titer and the SKOV-3 to HS-832 ratio was calculated.