Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2015 Sep 9.

Published in final edited form as: Curr Protoc Chem Biol. 2014 Sep 9;6(3):191–208. doi: 10.1002/9780470559277.ch140085

MALDI-MS profile of carbodiimide coupling modified N-glycans using GIG derived from 2 μL of human serum. After conjugation of serum proteins on beads and treated with EDC/p-toluidine, N-glycans released by PNGase F were directly analyzed using MALDI-MS without glycan purification. Only the structures of the major N-glycans are given. Symbol: solid blue square, N-acetylglucosamine; green circle, mannose; yellow circle, galactose; red triangle, fucose; purple diamond, N-acetyl-neuraminic acid.

MALDI-MS profile of carbodiimide coupling modified N-glycans using GIG derived from 2 μL of human serum. After conjugation of serum proteins on beads and treated with EDC/p-toluidine, N-glycans released by PNGase F were directly analyzed using MALDI-MS without glycan purification. Only the structures of the major N-glycans are given. Symbol: solid blue square, N-acetylglucosamine; green circle, mannose; yellow circle, galactose; red triangle, fucose; purple diamond, N-acetyl-neuraminic acid.