Figure 1.
Number of vertical jumps and horizontal shuttles between the cage and nest box after the injection of different doses (0, 5, 10, 20 mg/kg, IM) of morphine, which were considered as the vertical and horizontal activity indices, respectively. The number of vertical jumps after the first time (A), the second time (4 h after the first time) (B) and the third time (4 h after the second time) (C) administration of three doses of morphine. The number of horizontal shuttles between the cage and nest box after the first time (D), the second time (4 h after the first time) (E) and the third time (4 h after the second time) (F) administration of three doses of morphine. “*”, “#” and “+” represent, respectively, the significant difference between 5, 10 or 20 mg/kg and 0 mg/kg morphine. */#/+ p < 0.05, **/##/++ p < 0.01, and ***/###/+++ p < 0.001 vs. 0 mg/kg morphine. The data are expressed as means ± SEM, and analyzed using two-way ANOVA followed by Bonferroni post hoc test, n = 3.