Table 1. Experimental design for ERK inhibition experiment.
| Groups | First injection | Second injection | Injected amount (each time) | Animal # |
|---|---|---|---|---|
| IR + Saline |
0 h post IR |
6 h post IR |
4 μl saline |
3 |
| IR + DMSO |
0 h post IR |
6 h post IR |
4 μl 5% DMSO |
3 |
| IR + 0.4 mM U0126 |
0 h post IR |
6 h post IR |
4 μl 0.4 mM U0126 |
3 |
| IR + 0.8 mM U0126 | 0 h post IR | 6 h post IR | 4 μl 0.8 mM U0126 | 3 |