Figure 4.

Interleukin 6 (IL-6) and interleukin 1β (IL-1β) impair T-cell responses to interleukin 7 (IL-7). Peripheral blood mononuclear cells (PBMCs) from a healthy donor were treated in medium alone or supplemented with 1 ng/mL IL-6 or 10 ng/mL IL-1β for 2 days, followed by addition of 5 ng/mL IL-7. A, Stat-5 phosphorylation was measured 5 days after IL-7 addition. Representative and summary of 7 independent experiments are shown as means and standard errors. B, Surface staining for the α4β7 heterodimer 5 days after IL-7 addition. C, PBMCs from healthy donors were treated in medium alone or supplemented with 10 ng/mL IL-6 or 10 ng/mL IL-1β for 2 days, and intracellular Bcl2 expression was measured in CD4⁺ and CD8⁺ T cells 7 days after addition of IL-7 (2 ng/mL).