FIG 6 .

The viral factory is closely associated with the endoplasmic reticulum. (A) CV-1 cells were transfected with dsRedER-2 for 24 h before being infected with T3D at an MOI of 10 for 24 h. Following infection, cells were fixed and immunostained for μNS. The boxed area in each panel is enlarged to show detail (inset). Scale bars, 10 µm. (B) Cells were transfected and infected as described above with T1L or T3D. Following infection, cells were fixed and immunostained for σNS. The boxed area in each panel is enlarged to show detail (inset). (C) Cells were infected with T3D for 18 h before being fixed and immunostained for calnexin and anti-σNS. The boxed area in each panel is enlarged to show detail (inset).