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. 2014 Sep 23;5:487. doi: 10.3389/fmicb.2014.00487

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Copyright © 2014 Abdallah, Adel, Ouf, Sayed, Ghazy, Alam, Essack, Lafi, Bajic, El-Dorry and Siam.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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NMDS ordination plot based on KEGG mapping to methane metabolism pathways. Representation of the NMDS analysis in 3D (A–C) and 2D (D). The analysis considers each dataset of reads obtained through Red Sea interface shotgun pyrosequencing as a variable. The read counts recruited to methane metabolism-related KEGG orthologous groups were considered as an observation. The ordination of observations is represented by either blue triangles (cluster 1) or red squares (cluster 2). While the black crosses represent the ordination of variables, which is deduced from the weighted average of their respective observations. An ellipse around the centroid of each cluster depicts 95% confidence in the assignment of observations to the cluster.