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Flash-induced charge movements in the wild-type SRI–HtrI complex (black), the phototaxis signal-inverted H166S mutant (red), and the phenotypically wild-type H166S/R215W double mutant (green) at pH 7.0. Similar measurements were used to calculate the charge shift ratio of the same mutants by Sineshchekov et al.7 to establish the charge movement inversion and suppression effect confirmed here.
