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UvrABC incision of hairpin DNA (11) containing C4-AP ICL analogue 6. (A) Incision of 5′-32P-11: lane 1, nucleotide size markers; lane 2, unreacted 5′-32P-11; lane 3, incised 5′-32P-11; lane 4, A + G reaction; lane 5, T + C reaction. (B) Incision of internally labeled 32P-11: lane 1, nucleotide size markers; lane 2, unreacted 11; lane 3, incised 11; lane 4, 5′-32P-16.
