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. 2014 Sep 22;211(10):1977–1991. doi: 10.1084/jem.20132620

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© 2014 Rowland et al.

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Figure 4. — Antinuclear antibodies are reduced in BXSB.DTR mice after transient pDC depletion. Male DTR⁺ and DTR^– mice were treated with DT and analyzed at 19 wk of age. Untreated, healthy female littermates are shown. (A) Total IgG concentration in serum of indicated mice. (B) Antinuclear antibodies in sera were analyzed by HEp-2 assay. (left) Representative images are shown; (right) serum ANAs were titered to endpoint and Mann-Whitney test was applied. N.D. indicates signal was not detected at 1:40 dilution. (C) Concentration of IgG isotypes in serum of indicated mice was determined by ELISA. (D) Mean total serum immunoglobulin concentrations in indicated mice. (E) Sera from 19-wk-old male mice were subjected to an autoantigen microarray. Heat map of IgG antibody reactivity to indicated autoantigens in pDC-sufficient (BXSB.DTR^–, DT) and pDC-depleted (BXSB.DTR⁺, DT) mice is shown. Each column represents an individual mouse; each row indicates a single antigen. Relative maximum row signals are shown in red; relative minimum row signals are shown in blue. (F) Normalized signal of serum IgG reactivity for indicated antigens, as analyzed by autoantigen microarray described in (E). Data points indicate individual mice grouped from 10 experiments. Bar indicates arithmetic mean. *, P < 0.5; **, P < 0.01; ***, P < 0.001. Absence of * indicates nonsignificant differences.