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. Author manuscript; available in PMC: 2015 Mar 17.
Published in final edited form as: Nat Commun. 2014 Sep 17;5:4935. doi: 10.1038/ncomms5935

Figure 2. SMBAs block Bax phosphorylation and enhance apoptosis.

Figure 2

(a) A549 cells were metabolically labeled with [32P]orthophosphoric acid and treated with nicotine (1 μM) in the absence or presence of 5 μM of SMBA1, SMBA2 or SMBA3 for 60 min. Bax phosphorylation was determined by autoradiography (full blots can be found in Supplementary Fig. 6) (b) A549 cells were treated with cisplatin (40 μM), SMBA(s) (5 μM) or in combination in the absence or presence of nicotine (1 μM) for 48h. Cell viability was analyzed by PI-Annexin-V staining with FACS analysis. The error bars indicate ± SD of three separate experiments.