Table 1.

Inhibition of human sEH by substituted oxyoxalamide derivatives

No.	R	R1	R2	Human sEH IC50 (nM)a
3			H	>10,000
4			H	>10,000
5			H	838
6			H	50
7			H	>10,000
8			H	1500
9			H	>10,000
10			H	2300
11			H	4400
12			H	550
13			CH3	3200
14		CH3	CH3	>10,000
15			H	>10,000

^aTest compounds prepared in DMSO were reacted with human sEH (1 nM) for 10 min in 25 mM Bis–Tris/HCl buffer (202 μL; pH 7.0) at 30 °C. The fuorescent substrate (CMNPC; [S] = 5 μM) was then introduced to the incubation mixture. Inhibition potency against the human sEH was determined by measuring the appearance of the 6-methoxy-2-naphthaldehyde with an excitation wavelength of 330 nm and an emission wavelength of 465 nm for 10 min on a fluorometer. Results are averages of three separate measurements. See the Supplementary data for the detailed procedures.