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. Author manuscript; available in PMC: 2014 Sep 23.
Published in final edited form as: Leuk Lymphoma. 2013 Sep 10;55(4):899–910. doi: 10.3109/10428194.2013.819573

Figure 3. Comparison of mRNA expression of Bcl-2 family anti-apoptotic members in CLL cells between suspension cultures and stroma co-cultures (B–F).

Figure 3

Figure 3

CLL lymphocytes were incubated with NKTert stromal cell line at different time periods (12 hr, day 1, and day 3) and the individual transcript levels of Bcl-2 family proteins were determined using ‘human apoptotic array micro fluidic card’ (Applied Biosystems, 4378701) for Real-time PCR. A schematic illustration of different Bcl-2 family members is provided (A). mRNA expressions of a spectrum of anti-apoptotic Bcl-2 family proteins (B–F), were evaluated. Each symbol on the graph represents fold change in mRNA expression with respect to that after stromal co-incubations, which is normalized to respective mRNA levels of control samples. One sample t test statistical analysis was performed (*P<0.05, **P<0.01, ***P<0.001, ****P<0.0001).