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. Author manuscript; available in PMC: 2014 Sep 23.
Published in final edited form as: Leuk Lymphoma. 2013 Sep 10;55(4):899–910. doi: 10.3109/10428194.2013.819573

Figure 4. Comparison of mRNA expression of Bcl-2 family pro-apoptotic members in CLL between suspension cultures and stroma co-cultures (A–H).

Figure 4

Figure 4

CLL lymphocytes were incubated with NKTert stromal cell line at different time periods (12 hr, day 1, and day 3) and the individual transcript levels of pro-apoptotic Bcl-2 family proteins were determined using ‘human apoptotic array micro fluidic card’ (Applied Biosystems, 4378701) for Real-time PCR. mRNA levels of multi-domain pro-apoptotic Bcl-2 family members (A and B) and mRNA expressions of BH3-only pro-apoptotic proteins (C–H) were evaluated. Each symbol on the graph represents fold change in mRNA expression with respect to that after stromal co-incubations, which is normalized to respective mRNA levels of control samples. One sample t test statistical analysis was performed (*P<0.05, **P<0.01, ***P<0.001, ****P<0.0001).