FIG. 2.
CCIM with MSCs and Tregs in the early post-transplant period induces stable hematopoietic chimerism without graft-versus-host disease (GVHD) in full major histocompatibility complex (MHC)-mismatched murine models. On day 0, recipients received 3×107 T-cell-depleted (TCD) bone marrow (BM) cells from MHC-mismatched C57BL/6 (H-2b) donors. On days+1 and +3 after bone marrow transplantation (BMT), recipients received 2×106 MSCs, 2×106 Treg cells, or 2×106 MSCs plus 2×106 Treg cells. (A) The splenocytes was collected at 7 days after BM transplantation to detect host-derived NK cells by staining with anti-H-2d, anti-CD3, and anti-CD49b. (B) To obtain peripheral blood mononuclear cells (PBMCs), leukocytes isolated from recipients of these all groups were stained with MHC class I (H-2b and H-2d) at 5 weeks post-transplantation. The bars show the ratios of C57BL6-originated cells (■) and BALB/c-originated cells (□). (C) Percentages of donor cells (H-2d) in recipients in each group were assessed by flow cytometry. Data are shown as the mean±SEM; results are representative of four independent experiments. *P<0.05; **P<0.01.