Table 1.
Experimental Group
| Group | Donor | Recipient | TBI, Gy | DST,×107 | CY, mg/kg | BM/TCD-BM | CY, 50 mg/kg | MSC, 2×106 | Treg, 2×106 | Chimera | Rejection | GVHD |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Experiment 1 | ||||||||||||
| A | C57BL/6 | BALB/c | 1.5 | 3 | 100 | TCD-BM 3×107 | − | − | − | 0/20 | 20/20 | 0/20 |
| B | C57BL/6 | BALB/c | 1.5 | 3 | 100 | TCD-BM 3×107 | − | − | − | 2/5 | 3/5 | 0/5 |
| C | C57BL/6 | BALB/c | 1.5 | 3 | 100 | TCD-BM 3×107 | − | − | + | 1/5 | 4/5 | 0/5 |
| D | C57BL/6 | BALB/c | 1.5 | 3 | 100 | TCD-BM 3×107 | − | + | + | 17/20 | 3/20 | 0/20 |
| Experiment 2 | ||||||||||||
| A | C57BL/6 | BALB/c | 1.5 | 3 | 100 | BM 2.5×107 | + | − | − | 3/7 | 4/7 | 0/7 |
| B | C57BL/6 | BALB/c | 1.5 | 3 | 100 | BM 2.5×107 | − | + | + | 6/7 | 1/7 | 0/7 |
| C | C57BL/6 | BALB/c | 1.5 | 3 | 100 | BM 2.5×107 | − | − | − | 7/7 | 0/7 | 7/7 |
BM, bone marrow; CY, cyclophosphamide; DST, donor-specific cell transfusion; GVHD, graft-versus-host disease; MSC, mesenchymal stem cell; TBI, total-body irradiation; TCD, T-cell-depleted; Treg, regulatory T cells.