Figure 11.

Expression patterns of Pdu-Hes6 and their schematic representations in Platynereis larvae and during posterior elongation (PE). Whole-mount in situ hybridization (WMISH) for the different stages indicated on each panel is shown. Pdu-Hes6 is first expressed in ectodermal patches at 24 h post fertilization (hpf) then restricted to the midline, the stomodeum, two brain cells in the dorsal side and ectodermal lateral cells that form a more or less continuous line in each segment of the 48-hpf larvae. At 72 hpf its expression is greatly reduced and only concerns the midline plus minor patches in each segment. During PE, Pdu-Hes6 is still expressed in the midline, but also in the ectodermal segment addition zone (SAZ) and in the whole segment epidermis. Expression in both the dorsal and ventral segment epidermis is in a striped fashion, with more intensive expression in the borders and boundaries of the segments. For the larvae, panels are ventral views (anterior is up). Expressions patterns during PE are shown for both sides (anterior is up), V = ventral, D = dorsal. Deeper ventral view (different focus plane) is highlighted in red for 48hpf larvae.