Figure 12.

Expression patterns of Pdu-Hes8 and their schematic representations in Platynereis larvae and during posterior elongation (PE). Whole-mount in situ hybridization (WMISH) for the different stages indicated on each panel is shown. Pdu-Hes8 expression is mainly restricted to the ectodermal cells, in lateral patches in the episphere and hyposphere of the 24hpf post fertilization (hpf) larvae. At 48 and 72 hpf, its expression is reduced in minor patches in each segment and in the stomodeum, plus brain cells and mesodermal segment addition zone (SAZ) cells at 72 hpf. During PE, Pdu-Hes8 is expressed in the midline, and also in the whole segment epidermis, in the ectodermal SAZ and at the basis of the anal cirri. Expression in both the dorsal and ventral segment epidermis is in a striped fashion, with more intense expression in the borders and boundaries of the segments. For the larvae, panels are ventral views (anterior is up). Expressions patterns during PE are shown for both sides (anterior is up), V = ventral, D = dorsal.