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. 2014 Sep 5;5:29. doi: 10.1186/2041-9139-5-29

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Copyright © 2014 Gazave et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Expression patterns of Pdu-Hes12 and their schematic representations in Platynereis larvae and during posterior elongation (PE). Whole-mount in situ hybridization (WMISH) for the different stages indicated on each panel is shown. At 33 h post fertilization (hpf), Pdu-Hes12 is expressed in two large lateral patches that become restricted in a weak expression in the ventral nerve cord (VNC) and around the stomodeum at 48 hpf. At this stage, as well as during PE, this gene is also expressed in a single cell at the bottom of each chaetal sac. An expression in the stomodeum is also evidenced at 48 and 72 hpf. Panels are ventral views (anterior is up) for the larvae and during PE. Deeper ventral view (different focus plane) is highlighted in red for 48-hpf larvae.