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. 2014 Sep 5;5:29. doi: 10.1186/2041-9139-5-29

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Copyright © 2014 Gazave et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Expression patterns of Pdu-Hes3 and their schematic representations in Platynereis larvae and during posterior elongation (PE). Whole-mount in situ hybridization (WMISH) for the different stages indicated on each panel is shown. In the 48 and 72 h post-fertilization (hpf) trochophore larvae, Pdu-Hes3 is expressed in the mesoderm of the (presumptive) parapodia. During PE, Pdu-Hes3 expression covers the exterior borders of the ventral nerve cord (VNC), with a more large and intense expression in the VNC of the segments recently produced by the segment addition zone (SAZ). Pdu-Hes3 is also expressed in ectodermal patches at the basis of the chaetae. Panels are ventral views (anterior is up) for the larvae and during PE.