Table 1. Early embryonic but not adult-like HSCs prefer neonatal recipients.
LDA of reconstitution of neonatal and adult recipients with adult BM LT-HSCs, E14.5 FL LT-HSCs, whole E11.5 AGM, whole E10.5 AGM, and whole E9.5 PSp. The upper and lower limits provide the 95% confidence interval for the repopulating cell frequency. The goodness of fit tests how well the data fit the single-hit model that one repopulating cell will give a positive signal. A value of 1 suggests a good fit, less than 1 suggests heterogeneity in the donor cell population, and greater than 1 suggests a multi-hit model where recipients are hypersensitive to the given dose of donor cells. P-values are between neonatal and adult recipients transplanted with the same donor population and were calculated by the LDA algorithm. ND – not determined. Also see Table S1.
| Donor | Recipient | Helper Cell Dose |
Estimated Repopulating Cell Frequency |
Frequency Upper Limit |
Frequency Lower Limit |
Fit Single- hit Model |
p-value |
|---|---|---|---|---|---|---|---|
| BM LT-HSCs | Adult | 300,000 | 1/13.1 cells | 1/6.19 | 1/27.7 | 0.96 | 3.33E–8 |
| Neonate | None | 1/177 cells | 1/100 | 1/313 | 1.2 | ||
| FL LT-HSCs | Adult | 300,000 | 1/25.7 cells | 1/13.8 | 1/47.6 | 0.55 | 3.07E–4 |
| Neonate | None | 1/114 cells | 1/66.9 | 1/195 | 0.91 | ||
| E11.5 AGM | Adult | 20,000 | 1/0.538 ee | 1/0.24 | 1/1.2 | −4.46 | |
| 300,000 | 1/5.7 ee | 1/2.16 | 1/15 | 2.6 | 5.12E– 5 | ||
| Neonate | None | 1/0.935 ee | 1/0.624 | 1/1.4 | 0.74 | ||
| E10.5 AGM | Adult | ND | ND | ND | ND | ND | |
| Neonate | None | 1/2.84 ee | 1/1.6 | 1/5.02 | 0.71 | ||
| E9.5 PSp | Adult | ND | ND | ND | ND | ND | |
| Neonate | None | 1/44.8 ee | 1/18.4 | 1/109 | −0.212 |