Figure 1.

Real-time RT-PCR analysis of GRID1 mRNA levels in iPS clones. CDKL5 (33, 46, and 58)- and MECP2 (15)-mutated clones were analysed. BJ is a control clone derived from a healthy newborn male and clone 20 is a clone harbouring the CDKL5 mutation in one X chromosome but expressing the wild-type allele from the other X chromosome because of X-chromosome inactivation. The y axis shows 2^−ΔΔCt values (±SD). *P≤0.01; **P≤0.05; ***P≤0.001.