FIG. 2.

Pax6 mutation limits migration of embryoid body (EB) cells in vitro. (A) Pax6 mutation limited cellular migration away from EBs on a plastic substrate at 18 and 48 h after plating. A representative experiment is shown, and the experiment was repeated five times. 18 h scale bar=100 μm, 48 h scale bar=200 μm. (B) Cellular migration was evaluated by measuring the distance from the edge of the EBs to the nucleus of the most distant cell. Values are the mean±standard deviation (SD). (C) Transwell migration assay indicates that migration of cells from homozygous EBs (Homo-EBs) is blocked. Representative images of cells migrating to the lower chambers (lower cells) are presented. Scale bar=100 μm. (D) Number of migrated cells in transwell migration assays. A representative experiment is shown; the experiment was repeated three times. Each value represents the mean±SD (**P<0.01). Color images available online at www.liebertpub.com/scd