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. 2014 Apr 28;23(19):2297–2310. doi: 10.1089/scd.2013.0410

FIG. 3.

FIG. 3.

miR-124 inhibits migration of cells from Homo-EBs. (A) Immunostaining showing higher expression of IQGAP1 in wild-type EBs (Wt-EBs) (upper line) than in Homo-EBs (lower line). Scale bar=100 μm. (B) Western blotting showing higher expression of IQGAP1 in Wt-EBs than in Homo-EBs; β-actin was used as an internal control. (C) Relative miR-124 expression measured by quantitative polymerase chain reaction (qPCR). Quantitative analyses show that expression of miR-124 decreased in Wt-EBs but did not change in Homo-EBs. (D) Northern blotting showing expression of pre-miR-124 and mature miR-124 in iPS and EB cells. U6 was used as an internal control. (E) In situ hybridization showing miR-124 expression in Wt- and Homo-EBs at day 4 of culture. U6 and Scramble indicate the positive and negative controls, respectively. Scale bar=20 μm. A representative experiment is shown; the experiment was repeated three times. Each value represents the mean±SD (**P<0.01).