FIG. 4.

Overexpression of Pax6 results in decreased miR-124 expression in Homo-EBs. (A) Western blot result showing increased PAX6 expression after overexpression of Pax6 by Ad-PAX6-GFP infection in Homo-EBs. (B) Overexpression of Pax6 resulted in reduced miR-124 expression. qPCR analysis was performed independently for EBs (n=5). (C–E) Expression of miR-124 precursors was detected by qPCR. Significant changes were not observed for pre-miR-124-1 (C) or pre-miR-124-2 (D), whereas pre-miR-124-3 expression (E) was decreased in Pax6-overexpressing EBs (n=5). A representative experiment is shown; the experiment was repeated three times. Each value represents the mean±SD (**P<0.01). Color images available online at www.liebertpub.com/scd